ncRNA basic information
ncRNA ID:
MI0000103/MI0000739
ncRNA Database:
miRBase
ncRNA Name:
miR-101
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
RT-qPCR
ncRNA Target Gene:
VEGF-C
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
The expression levels of miRNA were determined by Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and those of protein were by Western blot analysis. Bioinformatics analyses were performed to predict the putative target genes of miR-101 using TargetScan software .Luciferase activity assay was used to verify the target genes of miRNAs. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs.
Drug Response:
sensitive
Cancer basic information
Cancer:
stomach cancer
Tissue/Cell:
cell line (SNU5, HGC27, BGC823, SGC7901,AGS,SGC7901/DDP,GES1)
Other information
Title:
MicroRNA-101 induces apoptosis in cisplatin-resistant gastric cancer cells by targeting VEGF-C.
Journal:
Mol Med Rep
Published:
2016
PubMed ID:
26573417