ncRNA basic information
ncRNA ID:
MI0000270/MI0000683
ncRNA Database:
miRBase
ncRNA Name:
miR-181b
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
Bim
ncRNA Pathway:
miR-181b-Bim-MMP-caspase pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00997 (APRD00185, DB05331, DB05847)
Drug Name:
Doxorubicin
Drug Method:
The expression levels of miRNA were determined by Quantitative real-time PCR (qRT-PCR) and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs.
Drug Response:
resistant
Cancer basic information
Cancer:
breast cancer
Tissue/Cell:
cell line (MCF-10A, T-47D, MCF-7, MDA-MB-231, MDA-MB-435)
Other information
Title:
MiR-181b promotes chemoresistance in breast cancer by regulating Bim expression.
Journal:
Oncol Rep
Published:
2016
PubMed ID:
26572075