ncRNA basic information
ncRNA ID:
MI0000083/MI0000750
ncRNA Database:
miRBase
ncRNA Name:
miR-26a
ncRNA Type:
miRNA
ncRNA Expression:
down-regulated
ncRNA Method:
Real-time RT-PCR
ncRNA Target Gene:
HMGA2
ncRNA Pathway:
E2F1-Akt pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
In this study, we analyzed expressional profiles of CDDP resistance-related mRNA, miRNA, and transcription factors (TF) that regulate miRNA expression in NSCLC. A549 cells were treated with CDDP, miR-26a mimic, or miR-26a inhibitor, and followed by biological analysis including drug sensitivity assay, colony formation assay, terminal-deoxynucleoitidyl Transferase Mediated Nick End Labeling (TUNEL) assay, and cell cycle analysis. Luciferase assay was used to determine the target of miR-26a. The regulation of miR-26a in Akt pathway was measured by western blot.
Drug Response:
resistant
Cancer basic information
Cancer:
lung non-small cell carcinoma
Tissue/Cell:
cell line (A549,A549/CDDPres )
Other information
Title:
Decreased MicroRNA-26a expression causes cisplatin resistance in human non-small cell lung cancer.
Journal:
Cancer Biol Ther
Published:
2016
PubMed ID:
26492332