ncRNA basic information
ncRNA ID:
MI0000242/MI0000281
ncRNA Database:
miRBase
ncRNA Name:
miR-199a
ncRNA Type:
miRNA
ncRNA Expression:
down-regulated
ncRNA Method:
RT-PCR
ncRNA Target Gene:
mTOR
ncRNA Pathway:
mTOR signaling pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
Real-time polymerase chain reaction(PCR) and Western blot were applied to detect the expression level changes of mammalian target of rapamycin (mTOR) in OV2008 and C13* cells before and after transfecting the cells with the mimics and inhibitor of miR-199a with Lipofectamine 2000.We constructed luciferase reporter vectors of mTOR and then transfected OV2008 cells with the mimics of miR-199a with Lipofectamine 2000 to study the regulation of miR-199as downstream target genes.
Drug Response:
resistant
Cancer basic information
Cancer:
ovarian cancer
Tissue/Cell:
cell line ( OV2008, C13* )
Other information
Title:
Involvement of miR-199a in cisplatin resistance of ovarian cancer cell through modulating expression of mTOR
Journal:
Zhonghua Yi Xue Za Zhi
Published:
2015
PubMed ID:
26711828