ncRNA basic information
ncRNA ID:
MI0000483
ncRNA Database:
miRBase
ncRNA Name:
miR-186
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
RT-PCR
ncRNA Target Gene:
ABCB1
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
The expression levels of miRNA were determined by Real-time reverse transcriptase-polymerase chain reaction (RT-PCR) and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs.
Drug Response:
sensitive
Cancer basic information
Cancer:
ovarian cancer
Tissue/Cell:
cell line (OVCAR3,A2780,A2780/DDP,A2780/Taxol,A2780/DDP, A2780/Taxol, OVCAR3)
Other information
Title:
MicroRNA-186 induces sensitivity of ovarian cancer cells to paclitaxel and cisplatin by targeting ABCB1.
Journal:
J Ovarian Res
Published:
2015
PubMed ID:
26626440