ncRNA basic information
ncRNA ID:
MI0000448
ncRNA Database:
miRBase
ncRNA Name:
miR-130a
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
BCL-2, MCL-1 and XIAP
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00619 (APRD01028, EXPT02967, DB03261)
Drug Name:
Imatinib
Drug Method:
We in this study aimed to investigate the function of miR130a in p53 tumor suppressor signaling pathway. We performed microRNA (miRNA) expression profile analysis in CML cancer stem cells of 38 cases and extracted total RNA from peripheral blood of 143 cases. Standard curves of U6 and miRNA were made from 10-fold serial dilutions of the cDNA, which were quantified using real-time quantitative PCR with SYBR Green by ABI 7300. The p53 mutations and BCR/ABL mutation status analysis in CML patients were detected by PCR and direct sequencing. Candidate targets of miR130a of putative relevance in CML pathogenesis were analyzed by bioinformatics approach. We then used dual-luciferase activity assay to verify the target genes of miR130a and used western blot analysis to elucidate the mechanism of miR130a on modulating drug resistance.
Drug Response:
sensitive
Cancer basic information
Cancer:
chronic myeloid leukemia
Tissue/Cell:
cell line
Other information
Title:
Functional studies of miR-130a on the inhibitory pathways of apoptosis in patients with chronic myeloid leukemia.
Journal:
Cancer Gene Ther
Published:
2015
PubMed ID:
26494558