ncRNA basic information
ncRNA ID:
MI0000446/MI0000470
ncRNA Database:
miRBase
ncRNA Name:
miR-125b
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
Real-time RT-PCR
ncRNA Target Gene:
Sema4C
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB01229 (APRD00259, DB05261, DB05927, DB05526, DB05281)
Drug Name:
Paclitaxel
Drug Method:
The expression levels of miRNA were determined by Real-time RT-PCR (RT-PCR) and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs. A wound healing assay was performed to examine the capacity of cell migration. Cell migration was detected by transwell invasion assay. Tumor xenograft model was used to test the role of miR-125b .
Drug Response:
sensitive
Cancer basic information
Cancer:
lung cancer
Tissue/Cell:
cell line (A549, H460 )
Other information
Title:
Up-regulation of miR-125b reverses epithelial-mesenchymal transition in paclitaxel-resistant lung cancer cells.
Journal:
Biol Chem
Published:
2015
PubMed ID:
26351908