ncRNA basic information
ncRNA ID:
MI0000449
ncRNA Database:
miRBase
ncRNA Name:
miR-132
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
RT-PCR
ncRNA Target Gene:
SIRT1
ncRNA Pathway:
SIRT1/CREB/ABCG2 signaling pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
The expression levels of miRNA were determined by Real-time PCR and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. Flow Cytometric Assay was used to determine if cells were viable, apoptotic, or necrotic. The CCK-8 assay was used to monitor the growth of the cells .
Drug Response:
resistant
Cancer basic information
Cancer:
stomach cancer
Tissue/Cell:
cell line (MKN45 and MKN28)
Other information
Title:
Upregulated miR-132 in Lgr5+ gastric cancer stem cell-like cells contributes to cisplatin-resistance via SIRT1/CREB/ABCG2 signaling pathway.
Journal:
Mol Carcinog
Published:
2017
PubMed ID:
28383763