ncRNA basic information
ncRNA ID:
MI0000443/MI0000444/MI0000445
ncRNA Database:
miRBase
ncRNA Name:
miR-124
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
real-time RT-PCR
ncRNA Target Gene:
ATMIN and PARP1
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00997 (APRD00185, DB05331, DB05847)
Drug Name:
Doxorubicin
Drug Method:
The expression levels of miRNA were determined by Real-time reverse transcription-PCR and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs. Potential direct miR-124 targets were predicted using the PicTar, TargetScan 5.1 and miRanda . The alkaline comet assay used a CometAssay kit .
Drug Response:
sensitive
Cancer basic information
Cancer:
osteosarcoma
Tissue/Cell:
cell line (MDA-MB-231, HCC1937, T-47D, ZR-75-1,MDA-MB-453, MCF-7, Hs 578T,MDA-MB-361)
Other information
Title:
The Effect of MicroRNA-124 Overexpression on Anti-Tumor Drug Sensitivity.
Journal:
PLoS One
Published:
2015
PubMed ID:
26115122