ncRNA basic information
ncRNA ID:
MI0000270/MI0000683
ncRNA Database:
miRBase
ncRNA Name:
miR-181b
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
RT-PCR
ncRNA Target Gene:
BCL2
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00544(APRD00516,EXPT03204)
Drug Name:
Fluorouracil
Drug Method:
The expression levels of miRNA were determined by real-time reverse transcription-polymerase chain reaction (RT-PCR) and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs.
Drug Response:
sensitive
Cancer basic information
Cancer:
lung cancer
Tissue/Cell:
cell line (SGC7901,SGC7901/VCR,A549,A549/CDDP)
Other information
Title:
miR-181b modulates multidrug resistance by targeting BCL2 in human cancer cell lines.
Journal:
Int J Cancer
Published:
2010
PubMed ID:
20162574