ncRNA basic information
ncRNA ID:
MI0000447/MI0000727
ncRNA Database:
miRBase
ncRNA Name:
miR-128
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
real-time RT-PCR
ncRNA Target Gene:
ZEB1
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
The miR-128 expression pattern of prostate cancer cell lines and tissues was detected by real-time reverse transcriptase-polymerase chain reaction, while the mRNA and protein expression levels of zinc-finger E-box-binding homeobox 1 were measured by real-time reverse transcriptase-polymerase chain reaction and western blot assay, respectively. Dual-luciferase reporter gene assay was used to find the direct target of miR-128. Furthermore, prostate cancer cells were treated with miR-128 mimic or zinc-finger E-box-binding homeobox 1-siRNA, and then the cells chemosensitivity and invasion were detected by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay and transwell assay, respectively.
Drug Response:
sensitive
Cancer basic information
Cancer:
prostate cancer
Tissue/Cell:
cell line (DU-145, LNCaP, HEK-293T)
Other information
Title:
miR-128 modulates chemosensitivity and invasion of prostate cancer cells through targeting ZEB1.
Journal:
Jpn J Clin Oncol
Published:
2015
PubMed ID:
25921099