ncRNA basic information
ncRNA ID:
MI0000266
ncRNA Database:
miRBase
ncRNA Name:
miR-10a
ncRNA Type:
miRNA
ncRNA Expression:
down-regulated
ncRNA Method:
qPCR
ncRNA Target Gene:
NA
ncRNA Pathway:
TGF-Beta/Smad2/STAT3/STAT5 pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
The expression levels of miRNA were determined by qPCR and those of protein were by Western blot analysis. MTS assay was used to test cell proliferation . Flow Cytometric Assay was used to determine if cells were viable, apoptotic, or necrotic. Transforming growth factor (TGF)-Beta detection was performed using an ELISA kit .
Drug Response:
sensitive
Cancer basic information
Cancer:
lung cancer
Tissue/Cell:
cell line (A549,A549/DDP )
Other information
Title:
MicroRNA-10a silencing reverses cisplatin resistance in the A549/cisplatin human lung cancer cell line via the transforming growth factor-Beta/Smad2/STAT3/STAT5 pathway.
Journal:
Mol Med Rep
Published:
2015
PubMed ID:
25586740