ncRNA basic information
ncRNA ID:
MI0000283
ncRNA Database:
miRBase
ncRNA Name:
miR-203
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qPCR
ncRNA Target Gene:
E2F3
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00853 (APRD00557)
Drug Name:
Temozolomide
Drug Method:
The expression levels of miRNA were determined by qPCR and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. A wound healing assay was performed to examine the capacity of cell migration. MTS assay was used to test cell proliferation . Cell migration was detected by transwell migration and invasion assay.
Drug Response:
sensitive
Cancer basic information
Cancer:
glioma
Tissue/Cell:
cell line (A172, U87MG, U251MG,HEK-293T)
Other information
Title:
MiR-203 sensitizes glioma cells to temozolomide and inhibits glioma cell invasion by targeting E2F3.
Journal:
Mol Med Rep
Published:
2015
PubMed ID:
25515700