ncRNA basic information
ncRNA ID:
MI0000070/MI0000115
ncRNA Database:
miRBase
ncRNA Name:
miR-16
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
Bcl-2
ncRNA Pathway:
PI3K/ Akt/ mTOR pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB01229 (APRD00259, DB05261, DB05927, DB05526, DB05281)
Drug Name:
Paclitaxel
Drug Method:
The expression levels of miRNA were determined by quantitative real-time PCR (qRT-PCR) and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs. Flow Cytometric Assay was used to determine if cells were viable, apoptotic, or necrotic.
Drug Response:
sensitive
Cancer basic information
Cancer:
lung cancer
Tissue/Cell:
cell line (Type II,A549, NCI-H596,NCI-H1734, NCI-H1299)
Other information
Title:
MiR-16 targets Bcl-2 in paclitaxel-resistant lung cancer cells and overexpression of miR-16 along with miR-17 causes unprecedented sensitivity by simultaneously modulating autophagy and apoptosis.
Journal:
Cell Signal
Published:
2015
PubMed ID:
25435430