ncRNA basic information
ncRNA ID:
MI0000082
ncRNA Database:
miRBase
ncRNA Name:
miR-25
ncRNA Type:
miRNA
ncRNA Expression:
down-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
CDC42
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
The expression levels of miRNA were determined by quantitative real-time polymerase chain reaction and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs. Lung cancer xenograft was used to test the role of miR-25 .
Drug Response:
sensitive
Cancer basic information
Cancer:
lung non-small cell carcinoma
Tissue/Cell:
cell line (H520, H460, A549,MRC5)
Other information
Title:
Downregulation of miR-25 modulates non-small cell lung cancer cells by targeting CDC42.
Journal:
Tumour Biol
Published:
2015
PubMed ID:
25432132