ncRNA basic information
ncRNA ID:
MI0000070/MI0000115
ncRNA Database:
miRBase
ncRNA Name:
miR-16
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
RT-qPCR
ncRNA Target Gene:
bcl-2
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00541 (APRD00495)
Drug Name:
Vincristine
Drug Method:
Mimics or inhibitors of miR-15a and miR-16 were transfected into HCT8 or HCT8/VCR cells with the help of Lipofectamine 2000. The expressions of miR-15a and miR-16 mRNA were detected by RT-qPCR. The levels of bcl-2 and P-gp proteins were measured by Western blot. The inhibitory effects of VCR on growth of HCT8 and HCT8/VCR cells were detected by CCK8.
Drug Response:
sensitive
Cancer basic information
Cancer:
colon cancer
Tissue/Cell:
cell line (HCT8, HCT8/VCR)
Other information
Title:
miR-15a and miR-16 modulate drug resistance by targeting bcl-2 in human colon cancer cells
Journal:
Zhonghua Zhong Liu Za Zhi
Published:
2014
PubMed ID:
25623762