ncRNA basic information
ncRNA ID:
MI0000299
ncRNA Database:
miRBase
ncRNA Name:
miR-222
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
RT-PCR
ncRNA Target Gene:
PUMA
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
The expression levels of miRNA were determined by RT-PCR and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs. Flow Cytometric Assay was used to determine if cells were viable, apoptotic, or necrotic.
Drug Response:
sensitive
Cancer basic information
Cancer:
oral squamous cell carcinoma
Tissue/Cell:
cell line (UM1)
Other information
Title:
MiR-222 targeted PUMA to improve sensitization of UM1 cells to cisplatin.
Journal:
Int J Mol Sci
Published:
2014
PubMed ID:
25474084