ncRNA basic information
ncRNA ID:
MI0000098
ncRNA Database:
miRBase
ncRNA Name:
miR-96
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
RECK
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
The expression levels of miRNA were determined by quantitative real-time PCR (qRT-PCR) and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs. Apoptosis was analyzed by flow cytometry using Annexin V/PI double staining. The CCK-8 assay was used to monitor the growth of the cells . Tumor formation assay in nude mouse models was used to test the role of miR-96 .
Drug Response:
sensitive
Cancer basic information
Cancer:
esophageal cancer
Tissue/Cell:
tissue and cell line (TE-1, ECa-109, EC-9706,HEEC)
Other information
Title:
MiR-96 promotes proliferation and chemo- or radioresistance by down-regulating RECK in esophageal cancer.
Journal:
Biomed Pharmacother
Published:
2014
PubMed ID:
25465153