ncRNA basic information ncRNA ID: MI0000066 ncRNA Database: miRBase ncRNA Name: let-7e ncRNA Type: miRNA ncRNA Expression: down-regulated ncRNA Method: qRT-PCR ncRNA Target Gene: NA ncRNA Pathway: NA Evidence (ncRNA-drug): validated

drug basic information Drug ID: DB00997 (APRD00185, DB05331, DB05847) Drug Name: Doxorubicin Drug Method: We created a doxorubicin-resistant MCF-7 (MCF-/Adr) cell line using a pulse-selection method; then verified the resistance of the MCF-7/Adr cell line to doxorubicin by using the methyl thiazolyl tetrazolium (MTT) assay, terminal deoxyribonucleotidyl transferase (TdT)-mediated dUTP nick-end labeling (TUNEL) staining, and Intracellular doxorubicin accumulation assay. Then, we performed qRT-PCR to detect the expression patterns of 14 selected miRNAs (which are related to breast cancer resistance) in both cell lines. Subsequently, we performed a bioinformatics analysis, including Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses, to determine the putative functions of 13 differentially expressed miRNA-targeted genes. Finally, we tested the expression levels of these 13 miRNAs in 10 chemotherapy non-responder breast cancer tissues and 29 responder tissues. All statistical analyses were performed by a two-tailed Students t-test, and a P value less than 0.05 was considered statistically significant. Drug Response: resistant

Cancer basic information Cancer: breast cancer Tissue/Cell: cell line (MCF-7,MCF-/Adr)

Other information Title: miRNA expression patterns in chemoresistant breast cancer tissues. Journal: Biomed Pharmacother Published: 2014 PubMed ID: 25451164