ncRNA basic information
ncRNA ID:
MI0000268
ncRNA Database:
miRBase
ncRNA Name:
miR-34a
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
NOTCH1
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00997 (APRD00185, DB05331, DB05847)
Drug Name:
Doxorubicin
Drug Method:
The expression levels of miRNA were determined by Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and those of protein were by Western blot analysis. Surface marker analysis by flow cytometry . Luciferase activity assay was used to verify the target genes of miRNAs. Caspase-3 activation status was measured using the Caspase-Glo 3 Assay . Lentiviral-mediated miRNA overexpression and xenograft was used to test the role of miR-34a .
Drug Response:
sensitive
Cancer basic information
Cancer:
breast cancer
Tissue/Cell:
cell line (MCF7, MCF7/ADR)
Other information
Title:
Targeting of miR34a-NOTCH1 axis reduced breast cancer stemness and chemoresistance.
Journal:
Cancer Res
Published:
2014
PubMed ID:
25368020