ncRNA basic information
ncRNA ID:
MI0003134
ncRNA Database:
miRBase
ncRNA Name:
miR-494
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
SCGN
ncRNA Pathway:
Bcl-2 pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
The expression levels of miRNA were determined by quantitative reverse transcription-PCR and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. The CCK-8 assay was used to monitor the growth of the cells . Flow Cytometric Assay was used to determine if cells were viable, apoptotic, or necrotic.
Drug Response:
sensitive
Cancer basic information
Cancer:
lung small cell carcinoma
Tissue/Cell:
cell line (NCI-H69, NCI-H446,NCI-H69AR)
Other information
Title:
Overexpression of secretagogin inhibits cell apoptosis and induces chemoresistance in small cell lung cancer under the regulation of miR-494.
Journal:
Oncotarget
Published:
2014
PubMed ID:
25226615