ncRNA basic information
ncRNA ID:
MI0000478
ncRNA Database:
miRBase
ncRNA Name:
miR-149
ncRNA Type:
miRNA
ncRNA Expression:
down-regulated
ncRNA Method:
qRT-PCR,RT-PCR
ncRNA Target Gene:
NSTD1
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB01229 (APRD00259, DB05261, DB05927, DB05526, DB05281)
Drug Name:
Paclitaxel
Drug Method:
The expression levels of miRNA were determined by quantitative real-time PCR and real-time PCR. Those of protein were by Western blot analysis.MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs. Targetscan online software was applied to predict the putative target gene of miR-149.
Drug Response:
resistant
Cancer basic information
Cancer:
breast cancer
Tissue/Cell:
cell line (MCF-7/WT, MCF-7/ADM, MCF-7/PTX, HCT-8/5-FU)
Other information
Title:
Methylation-regulated miR-149 modulates chemoresistance by targeting GlcNAc N-deacetylase/N-sulfotransferase-1 in human breast cancer.
Journal:
FEBS J
Published:
2014
PubMed ID:
25156775