ncRNA basic information
ncRNA ID:
MI0000475
ncRNA Database:
miRBase
ncRNA Name:
miR-136
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
E2F1
ncRNA Pathway:
E2F1-EZH2-caspase-3 signaling pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
The expression levels of miRNA were determined by quantitative real-time RT-PCR (qRT-PCR) and those of protein were by Western blot analysis. Cell migration was detected by transwell migration and invasion assay. MTS assay was used to test cell proliferation . Luciferase activity assay was used to verify the target genes of miRNAs. Targets gene analysis of miR-136 was performed by integrating the online databases .
Drug Response:
sensitive
Cancer basic information
Cancer:
glioma
Tissue/Cell:
tissue and cell line (U87, U251, U373)
Other information
Title:
MiR-136 targets E2F1 to reverse cisplatin chemosensitivity in glioma cells.
Journal:
J Neurooncol
Published:
2014
PubMed ID:
25139024