ncRNA basic information
ncRNA ID:
MI0000067
ncRNA Database:
miRBase
ncRNA Name:
let-7f-1
ncRNA Type:
miRNA
ncRNA Expression:
down-regulated
ncRNA Method:
RT-PCR
ncRNA Target Gene:
HMGB1
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
The expression levels of miRNA were determined by RT-PCR and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs. TUNEL staining was used to detect apoptotic cell death.
Drug Response:
resistant
Cancer basic information
Cancer:
medulloblastoma
Tissue/Cell:
cell line (D425,UW228)
Other information
Title:
miR-let-7f-1 regulates SPARC mediated cisplatin resistance in medulloblastoma cells.
Journal:
Cell Signal
Published:
2014
PubMed ID:
25014664