ncRNA basic information
ncRNA ID:
MI0000234
ncRNA Database:
miRBase
ncRNA Name:
miR-192
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
Bim
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
We performed miRNA microarray and RT-PCR to obtain the aberrant differential expressed miRNAs between A549 and its paired Cisplatin-resistant cell line A549/DDP cells, and then we investigated the biological functions of miR-192, which is the aberrant differential expressed miRNA. After transfection of the miR-192 into A549 cells, we measured the half inhibition concentration (IC50), cell apoptosis of the trasfectant cells, and then we used biological softwares and dual-luciferase report assay to explore the target gene of the miR-192, which was further validated by RT-PCR and Western blot.
Drug Response:
resistant
Cancer basic information
Cancer:
lung cancer
Tissue/Cell:
cell line (A549,A549/DDP)
Other information
Title:
MiR-192 confers cisplatin resistance by targeting Bim in lung cancer
Journal:
Zhongguo Fei Ai Za Zhi
Published:
2014
PubMed ID:
24854555