ncRNA basic information
ncRNA ID:
MI0003596
ncRNA Database:
miRBase
ncRNA Name:
miR-548b
ncRNA Type:
miRNA
ncRNA Expression:
down-regulated
ncRNA Method:
RT-qPCR
ncRNA Target Gene:
CCNB1
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00317 (APRD00997, DB07998)
Drug Name:
Gefitinib
Drug Method:
The expression levels of miRNA were determined by RT-qPCR and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. MTS assay was used to test cell proliferation . Flow Cytometric Assay was used to determine if cells were viable, apoptotic, or necrotic. Xenograft models in nude mice was used to test the role of miR-374a and miR-548b .
Drug Response:
sensitive
Cancer basic information
Cancer:
lung cancer
Tissue/Cell:
cell line (HCC827, Calu1)
Other information
Title:
Axl-altered microRNAs regulate tumorigenicity and gefitinib resistance in lung cancer.
Journal:
Cell Death Dis
Published:
2014
PubMed ID:
24832599