ncRNA basic information
ncRNA ID:
NA
ncRNA Database:
miRBase
ncRNA Name:
miR-200
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
RT-PCR
ncRNA Target Gene:
ERRFI-1
ncRNA Pathway:
EGFR pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00002 (BTD00071, BIOD00071)
Drug Name:
Cetuximab
Drug Method:
miRNA array screening and real-time reverse transcription-polymerase chain reaction were used to identify and validate the differential expression of miRNAs involved in EMT in 9 bladder cancer cell lines. A list of potential miR-200 direct targets was identified through the TargetScan database. The precursor of miR-200b and c were expressed in UMUC3 and T24 cells using a retrovirus or a lentivirus construct, respectively. Protein expression and signaling pathway modulation as well as intracellular distribution of EGFR and ERRFI-1 were validated through western blot analysis and confocal microscopy, whereas ERRFI-1 direct target of miR-200 members was validated by using the wild-type and mutanty 3'UTR/ERRFI-1/Luciferse reporters.
Drug Response:
sensitive
Cancer basic information
Cancer:
bladder cancer
Tissue/Cell:
cell line (UMUC,253J BV,KU7 )
Other information
Title:
miR-200 expression regulates epithelial-to-mesenchymal transition in bladder cancer cells and reverses resistance to epidermal growth factor receptor therapy.
Journal:
Clin Cancer Res
Published:
2009
PubMed ID:
19671845