ncRNA basic information
ncRNA ID:
MIMAT0000070
ncRNA Database:
miRBase
ncRNA Name:
miR-17-5p
ncRNA Type:
miRNA
ncRNA Expression:
down-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
beclin1
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB01229 (APRD00259, DB05261, DB05927, DB05526, DB05281)
Drug Name:
Paclitaxel
Drug Method:
In this study, we used microRNA (miRNA) arrays to screen differentially expressed miRNAs between paclitaxel sensitive lung cancer cells A549 and its paclitaxel-resistant cell variant (A549-T24). The expression levels of miRNA were determined by Quantitative Real-time PCR (qRT-PCR) and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. MTT assay was used to test the drug-resistant phenotype changes in cancer cells via overregulation of miRNAs. Trypan Blue Exclusion Assay for Cell Viability. Flow Cytometric Assay was used to determine if cells were viable, apoptotic, or necrotic.
Drug Response:
resistant
Cancer basic information
Cancer:
lung cancer
Tissue/Cell:
cell line (A549,NCI-H596)
Other information
Title:
miR-17-5p downregulation contributes to paclitaxel resistance of lung cancer cells through altering beclin1 expression.
Journal:
PLoS One
Published:
2014
PubMed ID:
24755562