ncRNA basic information
ncRNA ID:
MIMAT0000263
ncRNA Database:
miRBase
ncRNA Name:
miR-199b-5p
ncRNA Type:
miRNA
ncRNA Expression:
down-regulated
ncRNA Method:
quantitative real-time RT-PCR
ncRNA Target Gene:
JAG1
ncRNA Pathway:
JAG1-Notch1 signaling
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00515 (APRD00359)
Drug Name:
Cisplatin
Drug Method:
The expression levels of miRNA were determined by quantitative real-time RT-PCR and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. Cell viability was evaluated by XTT assay and Focus formation assay. in situ hybridization (ISH) was performed to evaluate the miR-199b-5p expression in a commercial tissue array (OVC1021, Pantomics) using the miRCURY LNA microRNA ISH Optimization Kit 5 (FFPE) .
Drug Response:
resistant
Cancer basic information
Cancer:
ovarian cancer
Tissue/Cell:
cell line (A2780s, A2780cp, OV2008, C13*,SKOV3, OVCA433, ES-2)
Other information
Title:
Epigenetic silencing of microRNA-199b-5p is associated with acquired chemoresistance via activation of JAG1-Notch1 signaling in ovarian cancer.
Journal:
Oncotarget
Published:
2014
PubMed ID:
24659709