ncRNA basic information
ncRNA ID:
MI0001733
ncRNA Database:
miRBase
ncRNA Name:
miR-452
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
RT-PCR
ncRNA Target Gene:
APC4
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB01248 (APRD00932)
Drug Name:
Docetaxel
Drug Method:
Real-time quantitative PCR (RT-qPCR) were used to identify the differential expression of miRNA-452 between MCF-7/DOC and MCF-7 cells. MiRNA-452 mimic was transfected into MCF-7 cells and miRNA-452 inhibitor was transfected into MCF-7/DOC cells. The role of miRNA-452 in these transfected cells was evaluated using RT-qPCR, MTT assay, and flow cytometry assay. The relationship of miRNA-452 and its predictive target gene anaphase-promoting complex 4 (APC4) was analyzed by RT-qPCR and Western blot.
Drug Response:
resistant
Cancer basic information
Cancer:
breast cancer
Tissue/Cell:
cell line (MCF-7,MCF-7/DOC)
Other information
Title:
MicroRNA-452 contributes to the docetaxel resistance of breast cancer cells.
Journal:
Tumour Biol
Published:
2014
PubMed ID:
24648265