ncRNA basic information
ncRNA ID:
MI0000650
ncRNA Database:
miRBase
ncRNA Name:
miR-200c
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
Real-time RT-PCR
ncRNA Target Gene:
ZEB1
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00441 (APRD00201)
Drug Name:
Gemcitabine
Drug Method:
In the present study, we compared the expression of miRNAs between gemcitabine-sensitive and gemcitabine-resistant pancreatic cancer cells and investigated whether the treatment of cells with natural agents [3,3-diindolylmethane (DIM) or isoflavone] could affect the expression of miRNAs. Moreover, we found that reexpression of miR-200 by transfection studies or treatment of gemcitabine-resistant cells with either DIM or isoflavone resulted in the down-regulation of ZEB1, slug, and vimentin, which was consistent with morphologic reversal of EMT phenotype leading to epithelial morphology.
Drug Response:
sensitive
Cancer basic information
Cancer:
pancreatic cancer
Tissue/Cell:
cell line (MiaPaCa-2, Panc-1, Aspc-1,L3.6pl, Colo357, BxPC-3, HPAC )
Other information
Title:
Up-regulation of miR-200 and let-7 by natural agents leads to the reversal of epithelial-to-mesenchymal transition in gemcitabine-resistant pancreatic cancer cells.
Journal:
Cancer Res
Published:
2009
PubMed ID:
19654291