ncRNA basic information
ncRNA ID:
MI0000102
ncRNA Database:
miRBase
ncRNA Name:
miR-100
ncRNA Type:
miRNA
ncRNA Expression:
up-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
HOXA1
ncRNA Pathway:
NA
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00997 (APRD00185, DB05331, DB05847)
Drug Name:
Doxorubicin
Drug Method:
The expression levels of miRNA were determined by quantitative reverse transcription polymerase chain reaction (qRT-PCR) and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. The CCK-8 assay was used to monitor the growth of the cells . Flow Cytometric Assay was used to determine if cells were viable, apoptotic, or necrotic.
Drug Response:
resistant
Cancer basic information
Cancer:
lung small cell carcinoma
Tissue/Cell:
cell line (NCI-H69,NCI-H69AR)
Other information
Title:
Downregulation of HOXA1 gene affects small cell lung cancer cell survival and chemoresistance under the regulation of miR-100.
Journal:
Eur J Cancer
Published:
2014
PubMed ID:
24559685