ncRNA basic information
ncRNA ID:
MI0000736/MI0000254
ncRNA Database:
miRBase
ncRNA Name:
miR-30c
ncRNA Type:
miRNA
ncRNA Expression:
down-regulated
ncRNA Method:
qRT-PCR
ncRNA Target Gene:
YWHAZ
ncRNA Pathway:
p38MAPK pathway
Evidence (ncRNA-drug):
validated
drug basic information
Drug ID:
DB00997 (APRD00185, DB05331, DB05847)
Drug Name:
Doxorubicin
Drug Method:
The expression levels of miRNA were determined by quantitative real-time PCR (qRT-PCR) and those of protein were by Western blot analysis. Luciferase activity assay was used to verify the target genes of miRNAs. The CCK-8 assay was used to monitor the growth of the cells . The bearing nude mouse model was used to identify the role of miR-30c .
Drug Response:
resistant
Cancer basic information
Cancer:
breast cancer
Tissue/Cell:
cell line (MCF-7, MDA-MB-231,MCF-7/ADR,MDA-MB-231/ADR)
Other information
Title:
Involvement of miR-30c in resistance to doxorubicin by regulating YWHAZ in breast cancer cells.
Journal:
Braz J Med Biol Res
Published:
2014
PubMed ID:
24519092